By Tomaz Jarm, Peter Kramar
This quantity offers the complaints of the first global Congress on Electroporation and Pulsed electrical Fields in Biology, medication and foodstuff & Environmental applied sciences (WC2015). The congress happened in Portorož, Slovenia, in the course of the week of September sixth to tenth, 2015. The medical a part of the Congress coated assorted points of electroporation and comparable applied sciences and incorporated the subsequent major subject matters:
· software of pulsed electrical fields know-how in nutrition: demanding situations and opportunities
· electric impedance size for evaluation of electroporation yield
· Electrochemistry and electroporation
· Electroporation meets electrostimulation
· Electrotechnologies for nutrients and biomass treatment
· nutrients and biotechnology applications
· In vitro electroporation - simple mechanisms
· Interfacial behaviour of lipid-assemblies, membranes and cells in electrical fields
· Irreversible electroporation in scientific use
· scientific functions: electrochemotherapy
· scientific purposes: gene therapy
· Non-electric field-based actual tools inducing telephone poration and more advantageous molecule transfer
· Non-thermal plasmas for nutrition safeguard, environmental functions and clinical treatments
· PEF for the meals undefined: basics and applications
· PEF proce
ss integration - advanced procedure chains and procedure mixtures within the meals industry
· Predictable animal models
Pulsed electrical fields and electroporation applied sciences in bioeconomy
· Veterinary scientific applications
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Extra resources for 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies: Portorož, Slovenia, September 6 –10, 2015
The focus of the lecture is to describe the basics of cell electropermeabilization and the different aspects of what is II. MECHANISMS OF MEMBRANE ELECTROPERMEABILIZATION AND DNA TRANSFER INTO CELLS. A. Basic Aspects: Modulation of Membrane Potential Difference The key effect of an electric field on cells is a positiondependent change in the resting transmembrane potential difference, ΔΨo, of their plasma membrane. The electrically induced potential difference, ΔΨE, which is the difference between the potential inside the cell, Ψin, and the potential outside the cell, Ψout, at a point M on the cell surface, is given by: ⎛ t ⎞⎤ ⎡ ⎜− ⎟ ΔΨE (t ) = Ψin − Ψout = −g(λ)rE cos(θM )⎢1− e⎝ τ ⎠⎥ (1) ⎢⎣ ⎥⎦ where t is the time after the onset of the electric pulse, g depends on the conductivities λ of the membrane, of the cytoplasm and of the extracellular medium, r is the radius of the cell, E the field strength, θM is the angle between the normal to the membrane at the position M and the direction of the field, and τ is the membrane charging time (14).
IFMBE Proceedings Vol. 53 Tissue Reactions to Electroporation and Electrochemotherapy: Vascular Effects that have Implications in Tumor Treatment 12. Bellard E, Markelc B, Pelofy S et al. (2012) Intravital microscopy at the single vessel level brings new insights of vascular modification mechanisms induced by electropermeabilization. J Control Release 163: 396-403. 13. Markelc B, Sersa G, Cemazar M (2013) Differential mechanisms associated with vascular disrupting action of electrochemotherapy: intravital microscopy on the level of single normal and tumor blood vessels.
L. Lukacs. 2006. Nucleocytoplasmic Transport of Plasmid DNA: A Perilous Journey from the Cytoplasm to the Nucleus. Hum Gene Ther 17:882-889. 27. Rosazza, C. et al. 2013. Intracellular tracking of single plasmid DNAparticles after delivery by electroporation. Mol Ther. 28. , D. C. Lamb, and C. Brauchle. 2011. Single-particle tracking as a quantitative microscopy-based approach to unravel cell entry mechanisms of viruses and pharmaceutical nanoparticles. Mol Ther 19:1199-1211. 29. Braeckmans, K. et al.
1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies: Portorož, Slovenia, September 6 –10, 2015 by Tomaz Jarm, Peter Kramar